4[piperidin-4-yliden-(3-carbamoylpheny)methyl]benzamide derivatives and their use for the treatment of pain spinal injuries or gastrointestinal disorders

ABSTRACT

Compounds of general formula (I) wherein R 1  is selected from any one of phenyl, pyridinyl, thienyl, furanyl, imidazolyl, pyrrolyl and triazolyl; where each R 1  phenyl ring and R 1  heteroaromatic ring may optionally and independently be further substituted by 1, 2 or 3 substituents selected from straight and branched C 1 –C 6  alkyl, NO 2 , CF 3 , C 1 –C 6  alkoxy, chloro, fluoro, bromo, and iodo. The substitutions on thephenyl ring and on the heteroaromatic ring may take place in any position on said ring systems; are disclosed and claimed in the present application, as well as their pharmaceutically acceptable salts and pharmaceutical compositions comprising the novel compounds and their use in therapy, inparticular in the management of pain.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a US National Stage of International Application No. PCT/SE02/01804 that was filed on Oct. 2, 2002. The International Application claims priority under 35 U.S.C. § 119(a) to Swedish Application No. 01033 13-3 filed Oct. 3, 2001.

FIELD OF THE INVENTION

The present invention is directed to novel compounds, to a process for their preparation, their use and pharmaceutical compositions comprising the novel compounds. The novel compounds are useful in therapy, and in particular for the treatment of pain.

BACKGROUND OF THE INVENTION

The δ receptor has been identified as having a role in many bodily functions such as circulatory and pain systems. Ligands for the δ receptor may therefore find potential use as analgesics, and/or as antihypertensive agents. Ligands for the δ receptor have also been shown to possess immunomodulatory activities.

The identification of at least three different populations of opioid receptors (μ, δ and κ) is now well established and all three are apparent in both central and peripheral nervous systems of many species including man. Analgesia has been observed in various animal models when one or more of these receptors has been activated.

With few exceptions, currently available selective opioid δ ligands are peptidic in nature and are unsuitable for administration by systemic routes. One example of a nonpeptidic δ-agonist is SNC80 (Bilsky E. J. et al., Journal of Pharmacology and Experimental Therapeutics, 273(1), pp. 359–366 (1995)). There is however still a need for selective δ-agonists having not only improved selectivity, but also an improved side-effect profile.

Thus, the problem underlying the present invention was to find new analgesics having improved analgesic effects, but also with an improved side-effect profile over current μ agonists, as well as having improved systemic efficacy.

Analgesics that have been identified and exist in the prior art have many disadvantages in that they suffer from poor pharmacokinetics and are not analgesic when administered by systemic routes. Also, it has been documented that preferred δ agonist compounds, described within the prior art, show significant convulsive effects when administered systemically.

We have now found that certain compounds not specifically disclosed by, but included within the scope of WO 98/28275, exhibit surprisingly improved δ-agonist properties and in vivo potency.

OUTLINE OF THE INVENTION

The novel compounds according to the present invention are defined by the formula I wherein

R¹ is selected from any one of (i) phenyl;

(ii) pyridinyl

(iii) thienyl

(iv) furanyl

(v) imidazolyl

(vi) triazolyl

(vii) pyrrolyl

and (viii) thiazolyl

where each R¹ phenyl ring and R¹ heteroaromatic ring may optionally and independently be further substituted by 1, 2 or 3 substituents independently selected from straight and branched C₁–C₆ alkyl, NO₂, CF₃, C₁–C₆ alkoxy, chloro, fluoro, bromo, and iodo. The substitutions on the phenyl ring and on the heteroaromatic ring may take place in any position on said ring systems.

Particularly, novel compounds according to the present invention are defined by the formula I

wherein R¹ is selected from any one of

(i) phenyl

(ii) pyridinyl

(iii) thienyl

and (iv) furanyl

Within the scope of the invention are also salts and enantiomers of the compounds of the formula I.

When the phenyl ring and the heteroaromatic ring(s) are substituted, the preferred substituents are independently selected from any one of CF₃, methyl, iodo, bromo, fluoro and chloro.

The novel compounds of the present invention are useful in therapy, especially for the treatment of various pain conditions such as chronic pain, neuropathic pain, acute pain, cancer pain, pain caused by rheumatoid arthritis, migraine, visceral pain etc. This list should however not be interpreted as exhaustive.

Compounds of the invention are useful as immunomodulators, especially for autoimmune diseases, such as arthritis, for skin grafts, organ transplants and similar surgical needs, for collagen diseases, various allergies, for use as anti-tumour agents and anti viral agents.

Compounds of the invention are useful in disease states where degeneration or dysfunction of opioid receptors is present or implicated in that paradigm. This may involve the use of isotopically labelled versions of the compounds of the invention in diagnostic techniques and imaging applications such as positron emission tomography (PET).

Compounds of the invention are useful for the treatment of diarrhoea, depression, anxiety, urinary incontinence, various mental illnesses, cough, lung oedema, various gastro-intestinal disorders, spinal injury and drug addiction, including the treatment of alcohol, nicotine, opioid and other drug abuse and for disorders of the sympathetic nervous system for example hypertension.

Compounds of the invention are useful as an analgesic agent for use during general anaesthesia and monitored anaesthesia care. Combinations of agents with different properties are often used to achieve a balance of effects needed to maintain the anaesthetic state (e.g. amnesia, analgesia, muscle relaxation and sedation). Included in this combination are inhaled anaesthetics, hypnotics, anxiolytics, neuromuscular blockers and opioids.

Also within the scope of the invention is the use of an), of the compounds according to the formula I above, for the manufacture of a medicament for the treatment of any of the conditions discussed above.

A further aspect of the invention is a method for the treatment of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to the formula I above, is admininstered to a patient in need of such treatment.

A further aspect of the present invention is intermediates of the general formula II

wherein PG is a urethane protecting group such as Boc or CBZ, or a benzyl or substituted benzyl protecting group, such as 2,4-dimethoxybenzyl.

Methods of Preparation

EXAMPLES

The invention will now be described in more detail by the following Schemes and Examples, which are not to be construed as limiting the invention.

Scheme 1: Synthetic Route to Compounds of the Present Invention

N,N-Diethyl-4-[N-Boc-piperidin-4-ylidene(3-carboxyphenyl)-methyl]-benzamide (2a)

A mixture of 4-[bromo-(4-diethylcarbamoyl-phenyl)-methylene]-piperidine-1-carboxylic acid tert-butyl ester (1a, 451 mg, 1.0 mmol), 3-carboxyphenyl boronic acid (330 mg, 2.0 mmol), 2M Na₂CO₃ (3 mL), and tetrakis(triphenyl phosphine) palladium(0) (25 mg) in toluene (degassed, 10 mL) and ethanol (degassed, 10 mL) was refluxed at 90° C. for 4 hrs under N₂. The reaction mixture was then quenched with aqueous NH₄Cl after cooling down to 0° C., and extracted with ethyl acetate (2×50 mL). The combined organic phases were washed with brine, dried over MgSO₄, and evaporated to give a crude product, which was purified by flash silica gel column to provide the desired compound 2a (345 mg, 70%): ¹H NMR (CDCl₃) δ 1.15 (3 H, br m, CH ₃CH₂—), 1.23 (3 H, br m, CH ₃CH₂—), 1.47 (9 H, s, C(CH₃)₃), 2.31 (2 H, m, piperidine CH—), 2.35 (2 H, m, piperidine CH—), 3.30 (2 H, br m, CH₃CH ₂N—), 3.48 (4 H, m, piperidine CH—), 3.54 (2 H, br m, CH₃CH ₂N—), 7.14 (2 H, d, J=8.0 Hz, ArH), 7.24 (1 H, m, ArH), 7.33 (2 H, d, J=8.0 Hz, ArH), 7.42 (1 H, t, J=7.6 Hz, ArH), 7.86(1 H, s, ArH), 7.97 (1 H, d, J=7.6 Hz, ArH). IR (NaCl)2976, 1718, 1691, 1598, 1430, 1233, 1166 cm⁻¹.

N,N-Diethyl-4-[1-benzyl-piperidin-4-ylidene-3-carboxyphenyl)-methyl]-benzamide (2b).

Method as for 2a using 1b (441 mg, 1.0 mmol) and 3-carboxyphenyl boronic acid (330 mg, 2.0 mmol) provided 2b (325 mg, 67%): ¹H NMR (CDCl₃) δ 1.11 (3 H, br m, CH ₃CH₂—), 1.22 (3 H, br m, CH ₃CH₂—), 2.63 (4 H, m, piperidine CH—), 2.90 (4 H, m, piperidine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 4.00 (2 H, s, CH ₂N—), 7.11 (2 H, d, J=8.0 Hz, ArH), 7.16 (1H, m, ArH), 7.30 (6 H, m, ArH), 7.42 (2 H, m, ArH), 7.84 (1 H, s, ArH), 7.96 (1 H, d, J=7.6 Hz, ArH).

N,N-Diethyl-4-[1-benzylpiperidin-4-ylidene(3-carbamoylphenyl)-methyl]-benzamide (3b).

241 mg (0.5 mmol) of N,N-diethyl-4-[piperidin-4-ylidene(3-carboxyphenyl)-methyl]-benzamide (2b), 780 mg (1.5 mmol) of PyBOP, and 200 mg (1.5 mmol) of HOBt were dissolved in 4 mL DMF. 0.58 mL (4 mmol) of DIPEA and 50 mg (10.0 mmol) of NH₄Cl were added successively. After stirred for 0:5 h at room temperature, the reaction mixture was quenched with water and ethyl ether. The white precipitates were collected as the desired product (3b, 152 mg, 63%): ¹H NMR (CDCl₃) δ 1.10 (3 H, br m, CH ₃CH₂—), 1.21 (3 H, br m, CH ₃CH₂—), 2.62 (4 H, m, piperidine CH—), 2.89 (4 H, m, piperidine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 4.00 (2 H, s, CH ₂N—), 7.11 (2 H, d, J=8.0 Hz, ArH), 7.16 (1H, m, ArH), 7.30(6 H, m, ArH), 7.42 (2 H, m, ArH), 7.84 (1 H, s, ArH), 7.96 (1 H, d, J=7.6 Hz, ArH); Anal.Calcd.for C₃₁H₃₅N₃O₂ 6.0 HCl: C, 53.16%; H, 5.90%; Found: C, 53.07%; H, 5.54%.

N,N-Diethyl-4-[piperidin-4-ylidene(3-carbamoylphenyl)-methyl]-benzamide (4)

Method as for 3b using 2a (150 mg, 0.30 mmol) provided 3a (105 mg, 70%): ¹H NMR (CDCl₃) δ 1.14 (3 H, br m, CH ₃CH₂—), 1.22 (3 H, br m, CH ₃CH₂—), 1.46 (9 H, s, C(CH3)3), 2.28 (2 H, m, piperidine CH—), 2.33 (2 H, m, piperidine CH—), 3.30 (2 H, br m, CH₃CH ₂N—), 3.46 (4 H, m, piperidine CH—), 3.55 (2 H, br m, CH₃CH ₂N—), 7.13 (2 H, d, J=8.0 Hz, ArH), 7.30 (3 H, m, ArH), 7.39(1 H, t, J=7.6 Hz, ArH), 7.61 (1 H, s, ArH), 7.68 (1 H, d, J=7.6 Hz, ArH).

The above product (3a) was treated with 4.0 M HCl in dioxane (10 mL) at room temperature for 4 h. After evaporation, the residue was dissolved in H₂O (10 mL) and impurities were extracted with ethyl acetate (2×20 mL). The aqueous phase was basified with NH₄OH and extracted with ethyl acetate (3×20 mL). The combined organic phases were washed with brine, dried over MgSO₄ and evaporated to give 4 in quantitative yield: ¹H NMR (CDCl₃) δ 1.15 (3 H, br m, CH ₃CH₂—), 1.23 (3 H, br m, CH ₃CH₂—), 1.90 (2 H, br, NH₂), 2.31 (2 H, m, piperidine CH—), 2.34 (2 H, m, piperidine CH—), 2.92 (4 H, m, piperidine CH—), 3.32 (2 H, br m, CH₃CH ₂N—), 3.55 (2 H, br m, CH₃CH ₂N—), 7.13 (2 H, d, J=8.0 Hz, ArH), 7.30 (3 H, m, ArH), 7.38 (1 H, t, J=7.6 Hz, ArH), 7.58 (1 H, s, ArH), 7.66 (1 H, d, J=7.6 Hz, ArH). IR (NaCl) 3307, 2973, 1668, 1615, 1435, 1383, 1289 cm⁻¹; Anal.Calcd.for C₂₄H₂₉N₃O₂ 2.8 HCl: C, 58.40%; H, 6.49%; Found: C, 58.46%;H, 6.57%.

N,N-Diethyl-4-[1-(2-thiophene)methyl-piperidin-4-ylidene-3-carbamoylphenyl)-methyl]-benzamide (5a)

To a mixture of N,N-diethyl-4-[piperidin-4-ylidene(3-carbamoylphenyl)-methyl]-benzamide (4, 196 mg, 0.5 mmol), 2-thiophenecarboxaldehyde (112 mg, 1.0 mmol), acetic acid (0.1 mL) in MeOH (10 mL) was added NaBH₃(CN) (200 mg) in portions. The reaction mixture was stirred for 4 h at room temperature, and then quenched with aqueous NH₄Cl, extracted with CH₂Cl₂ (3×50 mL). The combined organic phases were washed with brine, dried over MgSO₄ , and evaporated to give a crude product, which was purified by flash silica gel column to give the desired product (5a, 216 mg, 89%). ¹H NMR (CDCl₃) δ 1.13 (3 H, br m, CH ₃CH₂—), 1.22 (3 H, br m, CH ₃CH₂—), 2.36 (2 H, m, piperidine CH—), 2.42 (2 H, m, piperidine CH—), 2.54 (4 H, m, piperidine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 3.76 (2 H, s, CH ₂N—), 5.60 (1 H, br, NH), 6.06 (1 H, br, NH), 6.91 (1 H, s, ArH), 6.94 (1 H, m, ArH), 7.12 (2 H, d, J=8.0 Hz, ArH), 7.26 (1 H, m, ArH), 7.30 (3 H, m, ArH), 7.37 (1 H, t, J=7.6 Hz, ArH), 7.56 (1 H, s, ArH), 7.64 (1 H, d, J=7.6 Hz, ArH). IR (NaCl) 3352, 2973, 1668, 1614, 1434, 1290 cm⁻¹; Anal.Calcd.for C₂₉H₃₃N₃O₂S 2.3 HCl: C, 60.95%; H, 6.23%; Found: C, 60.96 %; H, 6.42%.

N,N-Diethyl-4-[1-(2-furfuryl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5b)

Method as for 5a using 4 (196 mg, 0.5 mmol) and 2-furaldehyde (96 mg, 1.0 mmol) provided 5b (158 mg, 67%): ¹H NMR (CDCl₃) δ 1.12 (3 H, br m, CH ₃CH₂—), 1.22 (3 H, br m, CH ₃CH₂—), 2.41 (4 H, m, piperidine CH—), 2.83 (4 H, m, piperidine CH—), 3.27 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 3.88 (2 H, s, CH ₂N—), 6.18 (1 H, br, NH), 6.35 (1 H, m, ArH), 6.42 (1 H, m, ArH), 6.86 (1 H, m, NH), 7.12 (2 H, d, J=8.0 Hz, ArH), 7.27 (3 H, m, ArH), 7.32 (1 H, m, ArH), 7.41 (1 H, s, ArH), 7.61 (1 H, s, ArH), 7.69 (1 H, d, J=7.6 Hz, ArH); Anal.Calcd.for C₂₉H₃₃N₃O₃ 3.0 HCl: C, 59.95%; H, 6.25%; Found: C, 59.68%; H, 5.98%.

N,N-Diethyl-4-[1-(3-furfuryl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5c)

Method as for 5a using 4 (196 mg, 0.5 mmol) and 3-furaldehyde (96 mg, 1.0 mmol) provided 5c (143 mg, 61%): ¹H NMR (CDCl₃) δ 1.13 (3 H, br m, CH ₃CH₂—), 1.25 (3 H, br m, CH₃CH ₂—), 2.42 (4 H, m, piperidine CH—), 2.70 (4 H, m, piperdine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 3.62 (2 H, s, CH ₂N—), 5.80 (1 H, br, NH), 6.42 (1 H, br, NH), 6.46 (1 H, s, ArH), 7.12 (2 H, d, J=8.0 Hz, ArH), 7.28 (3 H, m, ArH), 7.36 (1 H, t, J=7.6 Hz, ArH), 7.42 (2 H, m, ArH), 7.59 (1 H, s, ArH), 7.66 (1 H, d, J=7.6 Hz, ArH); Anal.Calcd.for C₂₉H₃₃N₃O₃ 3.1 HCl: C, 59.58%; H, 6.22%; Found. C, 59.44%; H, 6.45%.

N,N-Diethyl-4-[1-(2-pyridine)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5d)

Method as for 5a using 4 (196 mg, 0.5 mmol) and 2-pyridinecarboxaldehyde (107 mg, 1.0 mmol) provided 5d (35 mg, 15%): ¹H NMR (CDCl₃) δ 1.13 (3 H, br m, CH ₃CH₂—), 1.24 (3 H, br m, CH ₃CH₂—),2.38 (2 H, m, piperidine CH—), 2.42 (2 H, m, piperidine CH—), 2.56 (4 H, m, piperidine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.54 (2 H, br m, CH₃CH ₂N—), 3.68 (2 H, s, CH ₂N—), 5.64 (1 H, br, NH), 6.12 (1 H, br, NH), 7.14 (3 H, m, ArH), 7.31 (3 H, m, ArH), 7.35 (1 H, m, ArH), 7.38 (1 H, m, ArH), 7.56 (1 H, s, ArH), 7.65 (2 H, m, ArH), 8.58 (1 H, m, ArH).

N,N-Diethyl-4-[1-(3-thiophene)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5e)

Method as for 5a using 4 (196 mg, 0.5 mmol) and 3-thiophenecarboxaldehyde (112 mg, 1.0 mmol) provided 5e (185 mg, 76%): ¹H NMR (CDCl₃) δ 1.13 (3 H, br m, CH ₃CH₂—), 1.24 (3 H, br m, CHCH₂—), 2.46 (4 H, m, piperidine CH—), 2.84 (4 H, m, piperidine CH—), 3.26 (2 H, br m, CH₃CH ₂N—), 3.52 (2 H, br m, CH₃CH ₂N—), 3.91 (2 H, s, CH ₂N—), 5.72 (1 H, br, NH), 6.44 (1 H, br, NH), 7.13 (3 H, m, ArH), 7.26(3 H, m, ArH), 7.36 (3 H, m, ArH), 7.61 (1 H, s, ArH), 7.68 (1 H, d, J=7.6 Hz, ArH); Anal.Calcd.for C₂₉H₃₃N₃O₂S 2.5 HCl: C, 60.18%; H, 6.18 %; Found: C, 60.16%; H, 6.49%.

N,N-Diethyl-4-[1-(2-thiazole)methyl-piperidin-4 -ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5f)

To a solution of N,N-diethyl-4-[piperidin-4-ylidene (3-carbamoylphenyl)-methyl]-benzamide (4, 300 mg, 0.8 mmol) in 1,2-dichloroethane (15 mL) was added 2-thiazole carboxaldehyde (94 μL, 1.1 mmol) and sodium triacetoxyborohydride (228 mg, 1.1 mmol). The reaction mixture was stirred for 20 h at room temperature, and then quenched with aqueous NaHCO₃. The aqueous phase was extracted with CH₂Cl₂ (2×20 mL) and the combined organic phases were dried over MgSO₄, filtered and evaporated. The crude product was purified by flash chromatography to give product (5f) as a yellow foam (234 mg, 63% yield).

The product was dissolved in dichloromethane (5 mL) and a solution of HCl in ether (1N, 1.4 mL, 3eq.) was added. After 30 minutes the suspension was concentrated and the solid dried ¹H NMR (CDCl₃) δ 1.10 (3H, t, J=7 Hz, CH₃) ; 1.21 (3H, t, J=7 Hz, CH₃); 2.64 (4H, br s, CH₂); 3.27–3.31 (4H, m, CH₂); 3.48–3.53 (2H, m, CH₂); 3.67 (2H, br s, CH₂); 4.77 (2H, s, NCH₂Ar); 7.27 (2H, d, J=8.5 Hz, Ar—H) ; 7.33–7.37 (3H, m, Ar—H) ; 7.44 (1H, t, J=7.5 Hz, Ar—H); 7.68–7.69 (1H, m, Ar—H); 7.75–7.78 (2H, m, Ar—H); 7.94 (1H, d, J=3.5 Hz, Ar—H). Anal. Calcd for C₂₈H₃₂N₄O₂S×2.5HCl: C, 58.00%; H, 6.00%; N, 9.66%; Found: C, 58.00%; H, 5.95%; N, 9.43%.

N,N-Diethyl-4-[1-(3-pyridine)methyl-piperidin-4 -ylidene-(3-carbamoylphenyl)-methyl]-benzamide (5g)

Method as for 5f using 4 (176 mg, 0.45 mmol) and 3-pyridinecarboxyaldehyde (60 μL, 0.6 mmol) provided 5g (91.5 mg, 42%): ¹H NMR (CDCl₃) δ 1.09 (3H, t, J=7 Hz, CH₃); 1.21 (3H, t, J=7 Hz, CH₃); 2.68–2.75 (4H, m, CH₂); 3.26–3.28 (4H, m, CH₂); 3.43–3.60 (4H, m, CH₂); 4.65 (2H, s, NCH₂Ar); 7.27 (2H, d, J=8.5 Hz, Ar—H); 7.32–7.36 (3H, m, Ar—H); 7.43 (1H, t, J=8 Hz, Ar—H); 7.70–7.71 (1H, m, Ar—H); 7.75–7.78 (1H, m, Ar—H); 8.19 (1H, dd, J=6, 8 Hz, Ar—H); 8.88 (1H, d, J=8 Hz, Ar—H) ; 8.98 (1H, d, J=6 Hz, Ar—H); 9.21 (1H, s, Ar—H). Anal. Calcd for C₃₀H₃₄N₄O₂×3.1HCl×0.4H₂O: C, 59.77%; H, 6.34%; N, 9.29%; Found: C, 59.70%; H, 6.36%; N, 9.17%.

N,N-Diethyl-4-[1-(2-pyrrole)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)methyl ]-benzamide (5h)

Method as for 5f using 4 (261 mg, 0.7 mmol) and 2-pyrrolecarboxyaldehyde (89 mg, 0.9 mmol) provided 5h (118.5 mg, 38%): ¹H NMR (CDCl₃) δ 1.09 (3H, t, J=7 Hz, CH₃); 1.21 (3H, t, J=7 Hz, CH₃) ; 2.46–2.53 (2H, m, CH₂); 2.65–2.77 (2H, m, CH₂); 2.97–3.04 (2H, m, CH₂); 3.26–3.30 (2H, m, CH₂); 3.46–3.52 (4H, m, CH₂); 4.30 (2H, s, NCH₂Ar); 6.33–6.34 (1H, m, Ar—H); 6.85–6.86 (1H, m, Ar—H); 7.24–7.26 (2H, m, Ar—H); 7.30–7.36 (4H, m, Ar—H); 7.40–7.45 (1H, m, Ar—H); 7.67–7.68 (1H, m, Ar—H); 7.75–7.77 (1H, m, Ar—H). Anal. Calcd for C₂₉H₃₄N₄O₂×1.1HCl×1.8H₂O: C, 64.13%; H, 7.18%; N, 10.32%; Found: C, 64.26%; H, 7.15%; N, 9.94%.

N,N-Diethyl-4-[1-(4-pyridine)methyl-piperidin-4-ylidene-3-carbamoylphenyl)-methyl]-benzamide (5i)

Method as for 5f using 4 (329 mg, 0.8 mmol) and 4-pyridinecarboxyaldehyde (112 μL, 1.2 mmol) provided 5i (217 mg, 54%): ¹H NMR (CDCl₃) δ 1.12 (3H, t, J=7 Hz, CH₃); 1.24 (3H, t, J=7 Hz, CH₃); 2.67–2.82 (4H, m, CH₂); 3.22–3.34 (4H, m, CH₂); 3.49–3.65 (4H, m, CH₂); 4.72 (2H, s, NCH₂Ar); 7.29 (2H, d, J=8.5 Hz, Ar—H); 7.33–7.40 (3H, m, Ar—H); 7.46 (1H, t, J=8 Hz, Ar—H); 7.72–7.73 (1H, m, Ar—H); 7.78–7.80 (1H, m, Ar—H); 8.37 (2H, d, J=7 Hz, Ar—H); 9.00 (2H, d, J=7 Hz, Ar—H).

N,N-Diethyl-4-[1-(4-pyridine)methyl-piperidin-4-ylidene -(3-carbamoylphenyl)-methyl]-benzamide (5j)

Method as for 5f using 4 (313 mg, 0.8 mmol) and 4-imidazolecarboxyaldehyde (108 mg, 1.1mmol) provided 5j (68.2 mg, 18%): ¹H NMR (CDCl₃) δ 1.12 (3H, t, J=7 Hz, CH₃) ; 1.23 (3H, t, J=7 Hz, CH₃); 2.63 (2H, t, J=6 Hz, CH₂) ; 2.68 (2H, t, J=6 Hz, CH₂) ; 3.26–3.36 (6H, m, CH₂); 3.54 (2H, q, J=7 Hz, CH₂); 4.45 (2H, s, NCH₂Ar); 7.28 (2H, d, J=8 Hz, Ar—H); 7.32–7.40 (3H, m, Ar—H) ; 7.45 (1H, t, J=8 Hz, Ar—H); 7.66 (1H, s, Ar—H); 7.71 (1H, t, J=2 Hz, Ar—H); 7.76–7.82 (1H, m, Ar—H); 8.61 (1H, s, Ar—H).

Pharmaceutical Compositions

The novel compounds according to the present invention may be administered orally, intramuscularly, subcutaneously, topically, intranasally, intraperitoneally, intrathoracially, intravenously, epidurally, intrathecally, intracerebroventricularly and by injection into the joints.

A preferred route of administration is orally, intravenously or intramuscularly.

The dosage will depend on the route of administration, the severity of the disease, age and weight of the patient and other factors normally considered by the attending physician, when determining the individual regimen and dosage level as the most appropriate for a particular patient.

For preparing pharmaceutical compositions from the compounds of this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets, and suppositories.

A solid carrier can be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, or tablet disintegrating agents; it can also be an encapsulating material.

In powders, the carrier is a finely divided solid which is in a mixture with the finely divided active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.

For preparing suppository compositions, a low-melting wax such as a mixture of fatty acid glycerides and cocoa butter is first melted and the active ingredient is dispersed therein by, for example, stirring. The molten homogeneous mixture is then poured into convenient sized molds and allowed to cool and solidify.

Suitable carriers are magnesium carbonate, magnesium stearate, talc, lactose, sugar, pectin, dextrin, starch, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, a low-melting wax, cocoa butter, and the like.

Salts include, but are not limited to, pharmaceutically acceptable salts. Examples of pharmaceutically acceptable salts within the scope of the present invention include: acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate, bromide, calcium acetate, camsylate, carbonate, chloride, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, glucaptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, pamoate (embonate), pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, teoclate. Examples of pharmaceutically unacceptable salts within the scope of the present invention include: hydroiodide, perchlorate, and tetrafluoroborate.

Preferred pharmaceutically acceptable salts are the hydrochlorides, sulfates and bitartrates.

The hydrochloride and sulfate salts are particularly preferred.

The term composition is intended to include the formulation of the active component with encapsulating material as a carrier providing a capsule in which the active component (with or without other carriers) is surrounded by a carrier which is thus in association with it. Similarly, cachets are included. Tablets, powders, cachets, and capsules can be used as solid dosage forms suitable for oral administration.

Liquid from compositions include solutions, suspensions, and emulsions. Sterile water or water-propylene glycol solutions of the active compounds may be mentioned as an example of liquid preparations suitable for parenteral administration. Liquid compositions can also be formulated in solution in aqueous polyethylene glycol solution.

Aqueous solutions for oral administration can be prepared by dissolving the active component in water and adding suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous suspensions for oral use can be made by dispersing the finely divided active component in water together with a viscous material such as natural synthetic gums, resins, methyl cellulose, sodium carboxymethyl cellulose, and other suspending agents known to the pharmaceutical formulation art

Preferably the pharmaceutical composition is in unit dosage form. In such form, the composition is divided into unit doses containing appropriate quantities of the active component The unit dosage form can be a packaged preparation, the package containing discrete quantities of the preparations, for example, packeted tablets, capsules, and powders in vials or ampoules. The unit dosage form can also be a capsule, cachet, or tablet itself, or it can be the appropriate number of any of these packaged forms.

Biological Evaluation

In Vitro Model

Cell Culture

-   -   A. Human 293S cells expressing cloned human μ, δ, and κ         receptors and neomycin resistance were grown in suspension at         37° C. and 5% CO₂ in shaker flasks containing calcium-free DMEM         10% FBS, 5% BCS, 0.1% Pluronic F-68, and 600 μg/ml geneticin.     -   B. Mouse and rat brains were weighed and rinsed in ice-cold PBS         (containing 2.5 mM EDTA, pH 7.4). The brains were homogenized         with a polytron for 15 sec (mouse) or 30 sec (rat) in ice-cold         lysis buffer (50 mM Tris, pH 7.0, 2.5 mM EDTA, with         phenylmethylsulfonyl fluoride added just prior use to 0.5 MmM         from a 0.5M stock in DMSO:ethanol).         Membrane Preparation

Cells were pelleted and resuspended in lysis buffer (50 mM Tris, pH 7.0, 2.5 mM EDTA, with PMSF added just prior to use to 0.1 mM from a 0.1 M stock in ethanol), incubated on ice for 15 min, then homogenized with a polytron for 30 sec. The suspension was spun at 1000 g (max) for 10 min at 4° C. The supernatant was saved on ice and the pellets resuspended and spun as before. The supernatants from both spins were combined and spun at 46,000 g (max) for 30 min. The pellets were resuspended in cold Tris buffer (50 mM Tris/Cl, pH 7.0) and spun again. The final pellets were resuspended in membrane buffer (50 mM Tris, 0.32 M sucrose, pH 7.0). Aliquots (1 ml) in polypropylene tubes were frozen in dry ice/ethanol and stored at −70° C. until use. The protein concentrations were determined by a modified Lowry assay with sodium dodecyl sulfate.

Binding Assays

Membranes were thawed at 37° C., cooled on ice, passed 3 times through a 25-gauge needle, and diluted into binding buffer (50 mM Tris, 3 mM MgCl₂, 1 mg/ml BSA (Sigma A-7888), pH 7.4, which was stored at 4° C. after filtration through a 0.22 m filter, and to which had been freshly added 5 μg/ml aprotinin, 10 μM bestatin, 10μM diprotin A, no DTT). Aliquots of 100 μl were added to iced 12×75 mm polypropylene tubes containing 100 μl of the appropriate radioligand and 100 μl of test compound at various concentrations. Total (TB) and nonspecific (NS) binding were determined in the absence and presence of 10 μM naloxone respectively. The tubes were vortexed and incubated at 25° C. for 60–75 min, after which time the contents are rapidly vacuum-filtered and washed with about 12 ml/tube iced wash buffer (50 mM Tris, pH 7.0, 3 mM MgCl₂) through GF/B filters (Whatman) presoaked for at least 2 h in 0.1% polyethyleneimine. The radioactivity (dpm) retained on the filters was measured with a beta counter after soaking the filters for at least 12 h in minivials containing 6–7 ml scintillation fluid. If the assay is set up in 96-place deep well plates, the filtration is over 96-place PEI-soaked unifilters, which were washed with 3×1 ml wash buffer, and dried in an oven at 55° C. for 2 h. The filter plates were counted in a TopCount (Packard) after adding 50 μl MS-20 scintillation fluid/well.

Functional Assays

The agonist activity of the compounds is measured by determining the degree to which the compounds receptor complex activates the binding of GTP to G-proteins to which the receptors are coupled. In the GTP binding assay, GTP[γ]³⁵S is combined with test compounds and membranes from HEK-293S cells expressing the cloned human opioid receptors or from homogenised rat and mouse brain. Agonists stimulate GTP[γ]³⁵S binding in these membranes. The EC₅₀ and E_(max) values of compounds are determined from dose-response curves. Right shifts of the dose response curve by the delta antagonist naltrindole are performed to verify that agonist activity is mediated through delta receptors.

Data Analysis

The specific binding (SB) was calculated as TB-NS, and the SB in the presence of various test compounds was expressed as percentage of control SB. Values of IC₅₀ and Hill coefficient (n_(H)) for ligands in displacing specifically bound radioligand were calculated from logit plots or curve fitting programs such as Ligand, GraphPad Prism, SigmaPlot, or ReceptorFit. Values of K_(i) were calculated from the Cheng-Prussoff equation. Mean±S.E.M. values of IC₅₀, K_(i) and n_(H) were reported for ligands tested in at least three displacement curves. Biological data are tabulated on the following pages in Table 1.

TABLE 1 Biological data. MOLECULAR HDELTA (nM) RAT BRAIN MOUSE BRAIN Ex. # STRUCTURE IC₅₀ EC₅₀ % EMax EC₅₀ % EMax EC₅₀ % EMax 3b

0.548 0.091 93.775 0.403 178.94 0.539 164.82 5a

0.373 0.158 98.107 0.613 181.01 0.818 170.24 5b

0.282 0.633 149.28 5c

0.246 0.367 170.4 5d

0.278 0.396 179.89 5e

0.235 Receptor Saturation Experiments

Radioligand K₆₇ values were determined by performing the binding assays on cell membranes with the appropriate radioligands at concentrations ranging from 0.2 to 5 times the estimated K₆₇ (up to 10 times if amounts of radioligand required are feasible). The specific radioligand binding was expressed as pmole/mg membrane protein. Values of K₆₇ and B_(max) from individual experiments were obtained from nonlinear fits of specifically bound (B) vs. nM free (F) radioligand from individual according to a one-site model.

Determination of Mechano-Allodynia Using Von Frey Testing

Testing was performed between 08:00 and 16:00 h using the method described by Chaplan et al. (1994). Rats were placed in Plexiglas cages on top of a wire mesh bottom which allowed access to the paw, and were left to habituate for 10–15 min. The area tested was the mid-plantar left hind paw, avoiding the less sensitive foot pads. The paw was touched with a series of 8 Von Frey hairs with logarithmically incremental stiffness (0.41, 0.69, 1.20, 2.04, 3.63, 5.50, 8.51, and 15.14 grams; Stoelting, III, USA). The von Frey hair was applied from underneath the mesh floor perpendicular to the plantar surface with sufficient force to cause a slight buckling against the paw, and held for approximately 6–8 seconds. A positive response was noted if the paw was sharply withdrawn. Flinching immediately upon removal of the hair was also considered a positive response. Ambulation was considered an ambiguous response, and in such cases the stimulus was repeated.

Testing Protocol

The animals were tested on postoperative day 1 for the FCA-treated group. The 50% withdrawal threshold was determined using the up-down method of Dixon (1980). Testing was started with the 2.04 g hair, in the middle of the series. Stimuli were always presented in a consecutive way, whether ascending or descending. In the absence of a paw withdrawal response to the initially selected hair, a stronger stimulus was presented; in the event of paw withdrawal, the next weaker stimulus was chosen. Optimal threshold calculation by this method requires 6 responses in the immediate vicinity of the 50% threshold, and counting of these 6 responses began when the first change in response occurred, e.g. the threshold was first crossed. In cases where thresholds fell outside the range of stimuli, values of 15.14 (normal sensitivity) or 0.41 (maximally allodynic) were respectively assigned. The resulting pattern of positive and negative responses was tabulated using the convention, X=no withdrawal; O=withdrawal, and the 50% withdrawal threshold was interpolated using the formula: 50% g threshold=10^((Xf+kδ))/10,000 where Xf=value of the last von Frey hair used (log units); k=tabular value (from Chaplan et al. (1994)) for the pattern of positive /negative responses; and δ=mean difference between stimuli (log units). Here δ=0.224.

Von Frey thresholds were converted to percent of maximum possible effect (% MPE), according to Chaplan et al. 1994. The following equation was used to compute % MPE:

${\%\mspace{14mu}{MPE}} = \frac{{{Drug}\mspace{14mu}{treated}\mspace{14mu}{threshold}\mspace{14mu}(g)} - {{allodynia}\mspace{14mu}{{threshold}{\;\mspace{11mu}}(g)} \times 100}}{{{Control}\mspace{14mu}{{threshold}{\;\mspace{11mu}}(g)}} - {{allodynia}\mspace{14mu}{{threshold}{\mspace{11mu}\;}(g)}}}$ Administration of Test Substance

Rats were injected (subcutaneously, intraperitoneally, intravenously or orally) with a test substance prior to von Frey testing, the time between administration of test compound and the von Frey test varied depending upon the nature of the test compound

Writhing Test

Acetic acid will bring abdominal contractions when administered intraperitoneally in mice. These will then extend their body in a typical pattern. When analgesic drugs are administered, this described movement is less frequently observed and the drug selected as a potential good candidate.

A complete and typical Writhing reflex is considered only when the following elements are present: the animal is not in movement; the lower back is slightly depressed; the plantar aspect of both paws is observable. In this assay, compounds of the present invention demonstrate significant inhibition of writhing responses after oral dosing of 1–100 μmol/kg.

(i) Solutions Preparation

Acetic acid (AcOH): 120 μL of Acetic Acid is added to 19.88 ml of distilled water in order to obtain a final volume of 20 ml with a final concentration of 0.6% AcOH. The solution is then mixed (vortex) and ready for injection.

Compound (drug): Each compound is prepared and dissolved in the most suitable vehicle according to standard procedures.

(ii) Solutions Administration

The compound (drug) is administered orally, intraperitoneally (i.p.), subcutaneously (s.c.) or intravenously (i.v.)) at 10 ml/kg (considering the average mice body weight) 20, 30 or 40 minutes (according to the class of compound and its characteristics) prior to testing. When the compound is delivered centrally: Intraventricularly (i.c.v.) or intrathecally (i.t.) a volume of 5 μL is administered.

The AcOH is administered intraperitoneally (i.p.) in two sites at 10 ml/kg (considering the average mice body weight) immediately prior to testing.

(iii) Testing

The animal (mouse) is observed for a period of 20 minutes and the number of occasions (Writhing reflex) noted and compiled at the end of the experiment Mice are kept in individual “shoe box” cages with contact bedding. A total of 4 mice are usually observed at the same time: one control and three doses of drug. 

1. A compound of the formula I

wherein R¹ is selected from any one of (i) pyridinyl

(ii) thienyl

(iii) furanyl

(iv) imidazolyl

(v) triazolyl

(vi) pyrrolyl

 and (vii) thiazolyl

where each R¹ heteroaromatic ring may independently be further substituted by 1, 2 or 3 substituents independently selected from straight and branched C₁₋C₆alkyl, NO₂, CF₃, C₁–C₆ alkoxy, chloro, fluoro, bromo, and iodo; as well as salts thereof.
 2. A compound according to claim 1 wherein R¹ is selected from any one of (i) pyridinyl

(ii) thienyl

 and (iii) furanyl


3. A compound according to claim 1, wherein each R¹ heteroaromatic ring may optionally and independently be further substituted by 1, 2 or 3 substituents independently selected from methyl, CF₃, chloro, fluoro, bromo, and iodo.
 4. A compound according to claim 1, selected from any one of N,N-Diethyl-4-[1-(2-thiophene)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(2-furfuryl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(3-furfuryl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(2-pyridine)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(3-thiophene)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(2-thiazole)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(3-pyridine)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(2-pyrrole)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; N,N-Diethyl-4-[1-(4-pyridine)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide; and N,N-Diethyl-4-[1-(4-pyridine)methyl-piperidin-4-ylidene-(3-carbamoylphenyl)-methyl]-benzamide.
 5. A compound according to claim 1, in form of its hydrochloride, dihydrochloride, sulfate, tartrate, ditrifluoroacetate or citrate salts.
 6. A pharmaceutical composition comprising a compound of the formula I according to claim 1 as an active ingredient, together with a pharmaceutically acceptable carrier.
 7. A method for the treatment of pain, comprising administering to a subject in need of pain management an effective amount of a compound of the formula I according to claim
 1. 